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I really hope this helps! heres some extra ap bio resources that I found very helpful. good luck to everyone taking ap bio this yr. I know its hard but don't give up. resources: 5 steps to a 5 review book. very helpful to review units with and easy to read.Steps of the PCR Process. Denaturation (96°C): Heat the reaction strongly to separate, or denature, the DNA strands. This provides single-stranded DNA templates for the next step. Annealing (55 - 65°C): Cool the reaction so the primers can bind to their complementary sequences on the single-stranded template DNA.Learn for free about math, art, computer programming, economics, physics, chemistry, biology, medicine, finance, history, and more. Khan Academy is a nonprofit with the mission of providing a free, world-class education for anyone, anywhere.Metabolic pathways are enzyme-regulated sets of biochemical reactions that lead to either biosynthesis (anabolic pathways) or breakdown (catabolic pathways). 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